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KMID : 0364820200560010069
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Korean Journal of Microbiology
2020 Volume.56 No. 1 p.69 ~ p.71
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Draft genome sequence of Roseobacter sp. TSBP12 containing oxygenase activity to degrade indole
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Kim Hae-Seon
Cha Sun-Ho
Suk Ho-Young
Park Nyun-Ho
Woo Jung-Hee
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Abstract
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Roseobacter sp. TSBP12, a polyaromatic hydrocarbon (PAH) degrading bacterium, was isolated from an oil-contaminated sediment sample. This strain has been known to contain a strong oxidative/degradative activity of indole to produce indigo. In this study, the genomic structure of this strain was characterized using Ion Torrent PGM platform for the first time. A total of 96 contigs were generated, and the genome size was predicted to be 4,393,160 bp with the G + C content of 58.0%. Our analysis also revealed that this genome contained multiple oxygenases, including two subunits (a and b) of aromatic-ring hydroxylating dioxygenase and a flavin containing monooxygenase (FMO) that are known to be involved in the bioconversion of indigo from indole.
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KEYWORD
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Roseobacter, draft genome, indigo, indole, oxygenase activity
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